Analysis Note

ControlMouse brain

Application

This Anti-MAGI-2 Antibody is validated for use in IC, IH, IP, WB for the detection of MAGI-2.

General description

The spatial coordination of neurotransmitter receptors with other postsynaptic signaling and structural molecules is regulated by a diverse array of cell-specific scaffolding proteins. The synaptic trafficking of AMPA receptors by the stargazin protein in some neurons, for example, depends on specific interactions between the C terminus of stargazin and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of membrane-associated guanylate kinase scaffolding proteins PSD-93 or PSD-95. Stargazin [Cacng2 (Ca2+ channel gamma2 subunit)] is one of four closely related proteins recently categorized as transmembrane AMPA receptor regulating proteins (TARPs) that appear to share similar functions but exhibit distinct expression patterns in the CNS. MAGI-2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) has been identified as a novel candidate interactor with the cytoplasmic C termini of the TARPs. MAGI-2 [also known as S-SCAM (synaptic scaffolding molecule)] is a multi-PDZ domain scaffolding protein that interacts with several different ligands in brain, including PTEN (phosphatase and tensin homolog), dasm1 (dendrite arborization and synapse maturation 1), dendrin, axin, beta- and delta-catenin, neuroligin, hyperpolarization-activated cation channels, beta1-adrenergic receptors, and NMDA receptors. MAGI-2 coimmunoprecipitates with stargazin in vivo. Expression of stargazin recruites MAGI-2 to cell membranes and cell-cell contact sites in transfected HEK-293T cells dependent on the presence of the stargazin -TTPV motif. MAGI-2 represents a strong candidate for linking TARP/AMPA receptor complexes to a wide range of other postsynaptic molecules and pathways and advance our knowledge of protein interactions at mammalian CNS synapses.

Immunogen

GST-tagged MAGI-2

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Purified antibody in 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.

Quality

Routinely evaluated by Western Blotting on mouse brain tissue lysate.

Western Blotting Analysis: 1:500 dilution of this lot was used to detect MAGI-2 on mouse brain lysate.

Specificity

Cat. # AB9878 recognizes MAGI-2

Reactivity with other species has not been determined.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.

Target description

~140 kDa